The Proteomics Section of the Systems Technologies Core is led by Dr. Michael Bereman, Dept Biological Sciences, NC State.
The goal of the Proteomics Section is to provide CHHE members with expertise in experimental design, sample collection, preparation, and analysis for successful implementation of proteomics research. The Proteomics Section will enhance the ability of scientists working in the field of EHS to identify and capitalize on emerging opportunities in systems biology and the growing area of toxicoproteomics. Toxicoproteomics, a subfield of both proteomics and toxicogenomics, uses both global and targeted protein methodologies to identify and characterize critical proteins/complexes/pathways/receptors that are affected by, or respond to, chemical and environmental exposures. This section provides increased impact of CHHE members’ research results by: 1) correlating genome/epigenome studies with protein expression patterns; 2) discovering novel biomarkers of exposure in biological fluids and; 3) evaluating the impacts of exposure on key biological processes by characterizing protein expression, interaction, and modification using both in vitro cellular and in vivo animal models of exposure.
The Specific Aims of the Proteomics Section within the STC are:
AIM 1. Provide expertise in study design for proteomic experiments. The Proteomics Section provides guidance on the design of experiments, sample collection, protein extraction, protein solubilization, protein stabilization, protocol development, and data interpretation. The Section will provide reviews of relevant literature, when necessary, to educate members on novelty of and insight into less routine proteomic procedures.
AIM 2. Advance EHS research by implementing both discovery and targeted proteomic experiments. The Proteomics Section uses state-of-the-art LC MS/MS techniques to conduct discovery proteomic experiments, analyses of targeted proteins and their PTMs.
AIM 3. Provide consultation for members with projects that would benefit from other analytical measurement technologies. The Proteomics Section provides CHHE members direction (i.e., first point of contact) on the most appropriate analytical methodologies to address the needs of the experiment and the avenues to access these specific techniques (e.g. Metabolomics Section etc.).