The Proteomics Section of the Systems Technologies Core is led by Dr. Michael Bereman and Dr. Jeffrey Enders.
|Michael Bereman, PhD||
Jeffrey Enders, PhD
The Proteomics Section can consult on and conduct various proteomics-based experiments with CHHE members:
- Discovery proteomics: This workflow seeks to provide a qualitative inspection of the proteomic state of the sample(s) and is also capable of providing relative quantitation (i.e., sample A has more of protein X than sample B). Discovery proteomics could be considered a hypothesis-generating approach and therefore often leads to targeted proteomics.
- Targeted proteomics: This workflow seeks to identify and absolutely quantify a select number of proteins (via peptide analysis) and so requires a hypothesis at the outset.
- Post-translational modification (PTM): Proteins PTMs can have significant effects on protein structure and function. Detecting and localizing these PTMs in proteins using mass spectrometry can be very difficult, but is dependent on the magnitude of the mass shift due to the PTM, the abundance of the modified protein, the stability of the PTM, and the effect of the PTM on the peptide/protein’s ionization efficiency.
- Protein-protein interactions via immunoprecipitation (IP): IP studies provide a characterization of protein complexes and their response to regulatory mechanisms. The beads and antibodies used in these studies bind not only binding partners of the protein of interest, but also many non-specifically bound proteins. These factors necessitate a structured analysis strategy.
Please review our presentation below for more information on instruments and example studies that the Proteomics Section is able to assist with.
The Specific Aims of the Proteomics Section within the STC are:
AIM 1. Provide expertise in study design for proteomic experiments. The Proteomics Section provides guidance on the design of experiments, sample collection, protein extraction, protein solubilization, protein stabilization, protocol development, and data interpretation. The Section will provide reviews of relevant literature, when necessary, to educate members on novelty of and insight into less routine proteomic procedures.
AIM 2. Advance EHS research by implementing both discovery and targeted proteomic experiments. The Proteomics Section uses state-of-the-art LC MS/MS techniques to conduct discovery proteomic experiments, analyses of targeted proteins and their PTMs.
AIM 3. Provide consultation for members with projects that would benefit from other analytical measurement technologies. The Proteomics Section provides CHHE members direction (i.e., first point of contact) on the most appropriate analytical methodologies to address the needs of the experiment and the avenues to access these specific techniques (e.g. Metabolomics Section etc.).
Please contact Jeffrey Enders, PhD (firstname.lastname@example.org) for consultation and questions.