The Metals and Metallomics Section of the Systems Technologies Core (STC) is led by Dr. Dean Hesterberg. Dr. Hesterberg is the Metals/Metallomics Navigator for CHHE and all requests for consultation, support and services must go through the STC portal https://labs.ncsu.edu/link/SelectLab/STC for CHHE Project Requests
All CHHE Full Members are eligible for the CHHE’s new STC Voucher Program. Under this program, CHHE will provide 50% of the costs up to $15K/Full member for $30K STC services (for more information go to Services/Support and click on STC Voucher Program)
Metallomics involves the identification, roles, and impacts – both positive and negative – of metals and metalloids in biological systems. Although essential metal(loid)s such as Cu, Zn, Se, and Mn have specific, known biological functions, knowledge of uptake and toxicity mechanisms for these and non-essential metal(loid)s such as As, Pb, Cd, and Hg may require measurements of cellular distributions as well as specific metal complexation configurations in biological structures. The Metallomics Section will measure metals in environmental and biological samples.
The Aims of the Metals and Metallomics Section are:
Specific Aim 1: Provide expertise, guidance and consultation on experimental design for innovative -omics technologies involving metals and metallomics.
Dr. Hesterberg will to provide CHHE members with expertise in the characterization of metal/metalloid associations with biological structures (e.g., proteins, metabolites), including experimental design and analytical approaches for characterizing metal binding mechanisms and configurations. The Metallomics Section will focus on applying ICP-MS techniques such as LC-ICP-MS for liquids to gain information on metal(loid)s in biological systems.
Specific Aim 2: Perform metal analyses with state-of-the-art instrumentation and resources via a highly trained and qualified team.
All instrumentation in METRIC will be available for metallomics research, with the following instrument being most valuable for analysis of metals in biological systems, including bulk analysis of digested samples and speciation of metals associated with small molecules:
- Thermo Scientific ICAP RQ ICP-MS with Ultimate LC System and Elemental Scientific SC-2 DX Autosampler
Specific Aim 3: Perform and provide a seamless link to CHHE’s Environmental Data Science Group for training on analyses, advanced pathway mapping, data visualization and data storage.
Software, Data Analysis and Data Storage: Metallomics analyses will be performed by METRIC using software selected based on specific project requirements. Data can also be transferred to Bioinformatics Research Center- and CHHE-supported servers and analyzed by CHHE Environmental Data Science Group (EDSG) which brings together five bioinformaticians/biostatisticians. Bioinformatic and statistical support will be provided cost-free to CHHE Full members (see Bioinformatic Support section of IHSFC).